Protein Standards & Ladders - US (2024)

FAQs

What is a protein standard ladder? ›

Protein ladders, also known as protein markers or protein standards, are used to help estimate the size of proteins separated during electrophoresis. They serve as points of reference because they contain mixtures of highly purified proteins with known molecular weights and characteristics.

Protein standards are mixtures of well-characterized natural or recombinant proteins that are loaded alongside protein samples in a gel. They are used to help monitor electrophoretic separation as well as estimate the size and concentration of the proteins separated in a gel.

BenchMark Protein Ladder consists of 15 unstained recombinant proteins ranging in molecular weight from 10 to 220 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Fluorescent protein ladders are used in SDS-PAGE gels or polyacrylamide gel electrophoresis (involving tris-glycine buffer). They contain a mixture of proteins that are pre-stained with fluorescent dyes. The bands fluoresce when the light of a specific wavelength falls on them.

Thaw the ladder at room temperature. Do not boil protein ladder.

How much protein do I need a day? The Recommended Dietary Allowance (RDA) for protein is a modest 0.8 grams of protein per kilogram of body weight, or 0.36 grams per pound. The RDA is the amount of a nutrient you need to meet your basic nutritional requirements.

You can use this formula to calculate the amount of protein in your sample: [ (OD of the test sample) / (OD of the standard sample) ] X concentration of the standard sample. = 4.0 mg/mL protein (in test sample).

Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel. These standards contain pre-determined fragment (or protein) sizes and concentrations.

What is mass ladder? ›

Molecular weight size marker (ladder) is also referred to as a protein, DNA or RNA ladder. These ladders are a set of standards that are used to identify the approximate size of a molecule run on either an agarose or polyacrylamide gel during electrophoresis.

Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel.

For western blotting, use any of the SDS-PAGE ladders or those optimized for chemiluminescence detection. Whatever your needs for protein electrophoresis and western blotting, there is at least one protein ladder that meets all your requirements.

Purity: Look for ladders that are chromatography-purified to help ensure high purity and accurate results. Resolution: Choose a ladder with good resolution to easily resolve DNA fragments. Compatibility: Make sure the ladder is compatible with your gel system and run conditions, such as run time and voltage.

The major difference between native PAGE and SDS-PAGE is that in native PAGE, the protein migration rate is dependent on both the mass and structure, whereas in SDS-PAGE, the migration rate is determined only by protein's mass. In native PAGE, protein samples are prepared in a non-denaturing and non-reducing buffer.

A DNA ladder is a solution composed of DNA molecules of varying lengths. It is widely used in gel electrophoresis as a reference point or molecular-weight size marker to determine the approximate size of unknown DNA fragments.

Nucleotides are arranged in two long strands that form a spiral called a double helix. The structure of the double helix is somewhat like a ladder, with the base pairs forming the ladder's rungs and the sugar and phosphate molecules forming the vertical sidepieces of the ladder.

Transfer the desired amount of the Prestained Protein Ladder to a separate tube. For blotting: use 5 µl for mini-gels and 10 µl for full length gels. For visualizing during electrophoresis: use 10-15 µl for mini-gels and 20-30 µl for full length gels.

A DNA ladder, also known as a molecular size marker or standard DNA marker, is a solution containing DNA fragments of varying lengths and sizes that serves as a reference in estimating the size of unknown DNA molecules after they are separated by agarose or acrylamide gel electrophoresis.